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Shigeto Shimmura, MD, PhD

Biography_Shigeto_Shimmura_MD_PhD

Expertises:

Cornea, Regenerative Medicine

Current Affiliation:

Director, Fujita Health University Haneda Clinic

Professor, Department of Clinical Regenerative Medicine, Fujita Health University

Publications

  1. Hirayama M, Hatou S, Nomura M, Hokama R, Hirayama OI, Inagaki E, Aso K, Sayano T, Dohi H, Hanatani T, Takasu N, Okano H, Negishi K, Shimmura S. One year course of a first-in-human clinical study of an allogenic iPSC-derived corneal endothelial cell substitute transplantation for bullous keratopathy. Cell Reports Medicine. 2025;6(1):101847. doi:10.1016/j.celrep.2024.101847

  2. Rusch R, Inagaki E, Taniguchi H, Sakakura S, Tamai R, Nonaka H, Shimizu S, Sato S, Ogawa Y, Hirayama M, Negishi K, Okano H, Shimmura S. Adipose-Derived Mesenchymal Stromal Cells: A Study on Safety and Efficacy in Ocular Inflammation. The Ocular Surface. 2024. doi:10.1016/j.jcms.2024.11.002

  3. Sakakura S, Yamazaki R, Uchino Y, Negishi K, Shimmura S. Non-Descemet stripping endothelial keratoplasty for bullous keratopathy in patients with atopic dermatitis: A long-term case report. Medicine (Baltimore). 2024;103(39):e39665. doi:10.1097/MD.0000000000039665

  4. Asai K, Lee HK, Sato S, Shimizu E, Jung J, Okazaki T, Ogawa M, Shimmura S, Tsubota K, Ogawa Y, Negishi K, Hirayama M. The Necroptosis Pathway Is Upregulated in the Cornea in Mice With Ocular Graft-Versus-Host Disease. Invest Ophthalmol Vis Sci. 2024;65(10):38. doi:10.1167/iovs.65.10.38

  5. Sato S, Ogawa Y, Shimizu E, Asai K, Okazaki T, Rusch R, Hirayama M, Shimmura S, Negishi K, Tsubota K. Cellular senescence promotes meibomian gland dysfunction in a chronic graft-versus-host disease mouse model. Ocul Surf. 2024;32:198-210. doi:10.1016/j.jcms.2024.03.003

  6. Shimmura S, Inagaki E, Hirayama M, Hatou S. The Cornea: An Ideal Tissue for Regenerative Medicine. Keio J Med. 2024;73(1):1-7. doi:10.2302/kjm.73.1

  7. Sakakura S, Inagaki E, Ochiai Y, Yamamoto M, Takai N, Nagata T, Higa K, Sato Y, Toshida H, Murat D, Hirayama M, Ogawa Y, Negishi K, Shimmura S. A Comprehensive Assessment of Tear-Film-Oriented Diagnosis (TFOD) in a Dacryoadenectomy Dry Eye Model. Int J Mol Sci. 2023;24(22):16048. doi:10.3390/ijms242216048

  8. Altshuler A, Amitai-Lange A, Nasser W, Dimri S, Bhattacharya S, Tiosano B, Barbara R, Aberdam D, Shimmura S, Shalom-Feuerstein R. Eyes open on stem cells. Stem Cell Reports. 2023;18(12):2563-2565. doi:10.1016/j.stemcr.2023.11.002

  9. Sakakura S, Inagaki E, Sayano T, Yamazaki R, Fusaki N, Hatou S, Hirayama M, Tsubota K, Negishi K, Okano H, Shimmura S. Non-apoptotic regulated cell death in Fuchs endothelial corneal dystrophy. Regen Ther. 2023;24:592-601. doi:10.1016/j.reth.2023.08.002

  10. Terai H, Ishii M, Takemura R, Namkoong H, Shimamoto K, Masaki K, Tanosaki T, Chubachi S, Matsuyama E, Hayashi R, Shimada T, Shigematsu L, Ito F, Kaji M, Takaoka H, Kurihara M, Nakagawara K, Tomiyasu S, Sasahara K, Saito A, Otake S, Azekawa S, Okada M, Fukushima T, Morita A, Tanaka H, Sunata K, Asaoka M, Nishie M, Shinozaki T, Ebisudani T, Akiyama Y, Mitsuishi A, Nakayama S, Ogawa T, Sakurai K, Irie M, Yagi K, Ohgino K, Miyata J, Kabata H, Ikemura S, Kamata H, Yasuda H, Kawada I, Kimura R, Kondo M, Iwasaki T, Ishida N, Hiruma G, Miyazaki N, Ishibashi Y, Harada S, Fujita T, Ito D, Bun S, Tabuchi H, Kanzaki S, Shimizu E, Fukuda K, Yamagami J, Kobayashi K, Hirano T, Inoue T, Haraguchi M, Kagyo J, Shiomi T, Lee H, Sugihara K, Omori N, Sayama K, Otsuka K, Miyao N, Odani T, Watase M, Mochimaru T, Satomi R, Oyamada Y, Masuzawa K, Asakura T, Nakayama S, Suzuki Y, Baba R, Okamori S, Arai D, Nakachi I, Kuwahara N, Fujiwara A, Oakada T, Ishiguro T, Isosno T, Makino Y, Mashimo S, Kaido T, Minematsu N, Ueda S, Minami K, Hagiwara R, Manabe T, Fukui T, Funatsu Y, Koh H, Yoshiyama T, Kokuto H, Kusumoto T, Oashi A, Miyawaki M, Saito F, Tani T, Ishioka K, Takahashi S, Nakamura M, Harada N, Sasano H, Goto A, Kusaka Y, Ohba T, Nakano Y, Nishio K, Nakajima Y, Suzuki S, Yoshida S, Tateno H, Kodama N, Shunsuke M, Sakamoto S, Okamoto M, Nagasaki Y, Umeda A, Miyagawa K, Shimada H, Hagimura K, Nagashima K, Sato T, Sato Y, Hasegawa N, Takebayashi T, Nakahara J, Mimura M, Ogawa K, Shimmura S, Negishi K, Tsubota K, Amagai M, Goto R, Ibuka Y, Kitagawa Y, Kanai T, Fukunaga K. Comprehensive analysis of long COVID in a Japanese nationwide prospective cohort study. Respir Investig. 2023;61(6):802-814. doi:10.1016/j.resinv.2023.08.002

  11. Shimizu E, Sato S, Asai K, Ogawa Y, Shimmura S, Negishi K. Clinical Features of Sjogren Syndrome-Related Dry Eye Disease in Anterior Segment Photographs. Cornea. 2023;42(10):1314-1322. doi:10.1097/ICO.0000000000000407

  12. Hatou S, Shimmura S. Advances in corneal regenerative medicine with iPS cells. Jpn J Ophthalmol. 2023;67(5):541-545. doi:10.1007/s10384-023-01017-9

  13. Matsumae H, Yamaguchi T, Kusano Y, Shimmura S, Kobayashi A, Morizane Y, Shimazaki J. Graft Size and Double Scroll Formation Rate in Descemet Membrane Endothelial Keratoplasty. Curr Eye Res. 2022;47(9):1246-1251. doi:10.1080/02713683.2022.2070438

  14. Inagaki E, Arai E, Hatou S, Sayano T, Taniguchi H, Negishi K, Kanai Y, Sato Y, Okano H, Tsubota K, Shimmura S. The Anterior Eye Chamber as a Visible Medium for In Vivo Tumorigenicity Tests. Stem Cells Transl Med. 2022;11(8):841-849. doi:10.1002/sctm.22-0046

  15. Shimizu S, Sato S, Taniguchi H, Shimizu E, He J, Hayashi S, Negishi K, Ogawa Y, Shimmura S. Observation of Chronic Graft-Versus-Host Disease Mouse Model Cornea with In Vivo Confocal Microscopy. Diagnostics (Basel). 2021;11(8):1377. doi:10.3390/diagnostics11081377

  16. Rusch RM, Ogawa Y, Sato S, Morikawa S, Inagaki E, Shimizu E, Tsubota K, Shimmura S. MSCs Become Collagen-Type I Producing Cells with Different Phenotype in Allogeneic and Syngeneic Bone Marrow Transplantation. Int J Mol Sci. 2021;22(9):4870. doi:10.3390/ijms22094870

  17. Nakagawara K, Namkoong H, Terai H, Masaki K, Tano-saki T, Shimamoto K, Lee H, Tanaka H, Okamori S, Kabata H, Chubachi S, Ikemura S, Kamata H, Yasuda H, Kawada I, Ishii M, Ishibashi Y, Harada S, Fujita T, Ito D, Bun S, Tabuchi H, Kanzaki S, Shimizu E, Fukuda K, Yamagami J, Kobayashi K, Hirano T, Inoue T, Kagyo J, Shiomi T, Ohgino K, Sayama K, Otsuka K, Miyao N, Odani T, Oyamada Y, Masuzawa K, Nakayama S, Suzuki Y, Baba R, Nakachi I, Kuwahara N, Ishiguro T, Mashimo S, Minematsu N, Ueda S, Manabe T, Funatsu Y, Koh H, Yoshiyama T, Saito F, Ishioka K, Takahashi S, Nakamura M, Goto A, Harada N, Kusaka Y, Nakano Y, Nishio K, Tateno H, Edahiro R, Takeda Y, Kumanogoh A, Kodama N, Okamoto M, Umeda A, Hagimura K, Sato T, Miyazaki N, Takemura R, Sato Y, Takebayashi T, Nakahara J, Mimura M, Ogawa K, Shimmura S, Negishi K, Tsubota K, Amagai M, Goto R, Ibuka Y, Hasegawa N, Kitagawa Y, Kanai T, Fukunaga K. Comprehensive and long-term surveys of COVID-19 sequelae in Japan, an ambidirectional multicentre cohort study: study protocol. BMJ Open Respir Res. 2021;8(1):e001150. doi:10.1136/bmjresp-2021-001150

  18. Hatou S, Sayano T, Higa K, Inagaki E, Okano Y, Sato Y, Okano H, Tsubota K, Shimmura S. Transplantation of iPSC-derived corneal endothelial substitutes in a monkey corneal edema model. Stem Cell Res. 2021;55:102497. doi:10.1016/j.stemcr.2021.102497

  19. Hata-Mizuno M, Uchino Y, Uchino M, Shimmura S, Ogawa Y, Tsubota K, Negishi K. Analysis of the Association between Galectin-3 Concentration in Tears and the Severity of Dry Eye Disease: A Case-Control Study. J Clin Med. 2021;11(1):257. doi:10.3390/jcm11010257

  20. Yamazaki R, Nejima R, Ichihashi Y, Miyata K, Tsubota K, Shimmura S. Descemet stripping and automated endothelial keratoplasty (DSAEK) versus non-Descemet stripping and automated endothelial keratoplasty (nDSAEK) for bullous keratopathy. Jpn J Ophthalmol. 2020;64(6):585-590. doi:10.1007/s10384-020-00787-w

  21. Yamane M, Sato S, Shimizu E, Shibata S, Hayano M, Yaguchi T, Kamijuku H, Ogawa M, Suzuki T, Mukai S, Shimmura S, Okano H, Takeuchi T, Kawakami Y, Ogawa Y, Tsubota K. Senescence-associated secretory phenotype promotes chronic ocular graft-vs-host disease in mice and humans. FASEB J. 2020;34(8):10778-10800. doi:10.1096/fj.202000216R

  22. Higa K, Higuchi J, Kimoto R, Miyashita H, Shimazaki J, Tsubota K, Shimmura S. Human corneal limbal organoids maintaining limbal stem cell niche function. Stem Cell Res. 2020;49:102012. doi:10.1016/j.stemcr.2020.102012

  23. Hata-Mizuno M, Ingaki E, Mitamura H, Uchino Y, Tsubota K, Shimmura S. Conjunctival Epithelial Ingrowth After Penetrating Keratoplasty. Cornea. 2020;39(9):1181-1183. doi:10.1097/ICO.0000000000002440

  24. Yamashita K, Hatou S, Uchino Y, Tsubota K, Shimmura S. Prognosis after lamellar keratoplasty for limbal dermoids using preserved corneas. Jpn J Ophthalmol. 2019;63(1):56-64. doi:10.1007/s10384-018-0628-8

  25. Venkatraman A, Hochart G, Bonnel D, Stauber J, Shimmura S, Rajamani L, Pervushin K, Mehta JS. Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging of Key Proteins in Corneal Samples from Lattice Dystrophy Patients with TGFBI-H626R and TGFBI-R124C Mutations. Proteomics Clin Appl. 2019;13(1):e1800053. doi:10.1002/prca.201800053

  26. Fujii S, Yoshida S, Inagaki E, Hatou S, Tsubota K, Takahashi M, Shimmura S, Sugita S. Immunological Properties of Neural Crest Cells Derived from Human Induced Pluripotent Stem Cells. Stem Cells Dev. 2019;28(1):28-43. doi:10.1089/scd.2018.0125

  27. Deng SX, Borderie V, Chan CC, Dana R, Figueiredo FC, Gomes JAP, Pellegrini G, Shimmura S, Kruse FE, G and The International Limbal Stem Cell Deficiency Working Group. Global Consensus on Definition, Classification, Diagnosis, and Staging of Limbal Stem Cell Deficiency. Cornea. 2019;38(3):364-375. doi:10.1097/ICO.0000000000001844

  28. Yamashita K, Inagaki E, Hatou S, Higa K, Ogawa A, Miyashita H, Tsubota K, Shimmura S. Corneal Endothelial Regeneration Using Mesenchymal Stem Cells Derived from Human Umbilical Cord. Stem Cells Dev. 2018;27(16):1097-1108. doi:10.1089/scd.2018.0051

  29. Yamashita K, Hatou S, Inagaki E, Higa K, Tsubota K, Shimmura S. A Rabbit Corneal Endothelial Dysfunction Model Using Endothelial-Mesenchymal Transformed Cells. Sci Rep. 2018;8(1):16868. doi:10.1038/s41598-018-35201-4

  30. Yamane M, Ogawa Y, Mukai S, Yaguchi S, Kamijuku H, Inaba T, Asai K, Morikawa S, Kawakami Y, Shimmura S, Tsubota K. Functional Role of Lacrimal Gland Fibroblasts in a Mouse Model of Chronic Graft-Versus-Host Disease. Cornea. 2018;37(1):102-108. doi:10.1097/ICO.0000000000001429

  31. Den S, Shimmura S, Shimazaki J. Cataract surgery after deep anterior lamellar keratoplasty and penetrating keratoplasty in age- and disease-matched eyes. J Cataract Refract Surg. 2018;44(4):496-503. doi:10.1016/j.jcrs.2018.01.031

  32. Yamazaki R, Yamazoe K, Yoshida S, Hatou S, Inagaki E, Okano H, Tsubota K, Shimmura S. The Semaphorin 3A inhibitor SM-345431 preserves corneal nerve and epithelial integrity in a murine dry eye model. Sci Rep. 2017;7(1):15584. doi:10.1038/s41598-017-15579-2

  33. Shimmura-Tomita M, Shimmura S, Tsubota K, Shimazaki J. Penetrating Keratoplasty Performed by Residents Compared With an Experienced Cornea Transplant Surgeon. J Surg Educ. 2017;74(2):258-263. doi:10.1016/j.jsurg.2016.10.005

  34. Miyashita H, Niwano H, Yoshida S, Hatou S, Inagaki E, Tsubota K, Shimmura S. Long-term homeostasis and wound healing in an in vitro epithelial stem cell niche model. Sci Rep. 2017;7:43557. doi:10.1038/srep43557

  35. Inagaki E, Hatou S, Higa K, Yoshida S, Shibata S, Okano H, Tsubota K, Shimmura S. Skin-Derived Precursors as a Source of Progenitors for Corneal Endothelial Regeneration. Stem Cells Transl Med. 2017;6(3):788-798. doi:10.1002/sctm.16-0180

  36. Hirayama M, Ko SBH, Kawakita T, Akiyama T, Goparaju SK, Soma A, Nakatake Y, Sakota M, Chikazawa-Nohtomi N, Shimmura S, Tsubota K, Ko MSH. Identification of transcription factors that promote the differentiation of human pluripotent stem cells into lacrimal gland epithelium-like cells. NPJ Aging Mech Dis. 2017;3:1. doi:10.1038/s41514-017-0002-z

  37. Ogawa Y, Morikawa S, Okano H, Mabuchi Y, Suzuki S, Yaguchi T, Sato Y, Mukai S, Yaguchi S, Inaba T, Okamoto S, Kawakami Y, Tsubota K, Matsuzaki Y, Shimmura S. MHC-compatible bone marrow stromal/stem cells trigger fibrosis by activating host T cells in a scleroderma mouse model. Elife. 2016;5:e09394. doi:10.7554/eLife.09394

  38. Ogawa A, Matsumoto Y, Yaguchi T, Shimmura S, Tsubota K. Successful treatment of Beauveria bassiana fungal keratitis with topical voriconazole. J Infect Chemother. 2016;22(4):257-260. doi:10.1016/j.jiac.2015.12.001

  39. Ishiyama S, Mori Y, Nejima R, Tokudome T, Shimmura S, Miyata K, Amano S. Comparison of Long-Term Outcomes of Visual Function and Endothelial Cell Survival After Descemet Stripping Automated Endothelial Keratoplasty and Penetrating Keratoplasty Using Mixed-Effects Models. Cornea. 2016;35(2):161-167. doi:10.1097/ICO.0000000000000729

  40. Hirayama M, Liu Y, Kawakita T, Shimmura S, Tsubota K. Cytokeratin expression in mouse lacrimal gland germ epithelium. Exp Eye Res. 2016;146:54-59. doi:10.1016/j.exer.2016.03.003

  41. Yamazoe K, Yoshida S, Yasuda M, Hatou S, Inagaki E, Ogawa Y, Tsubota K, Shimmura S. Development of a Transgenic Mouse with R124H Human TGFBI Mutation Associated with Granular Corneal Dystrophy Type 2. PLoS One. 2015;10(7):e0133397. doi:10.1371/journal.pone.0133397

  42. Yaguchi S, Ogawa Y, Kawakita T, Shimmura S, Tsubota K. Tissue Renin-Angiotensin System in Lacrimal Gland Fibrosis in a Murine Model of Chronic Graft-Versus-Host Disease. Cornea. 2015;34 Suppl 11:S142-152. doi:10.1097/ICO.0000000000000572

  43. Kawakita T, Shimmura S, Tsubota K. Effect of Oral Pilocarpine in Treating Severe Dry Eye in Patients With Sjogren Syndrome. Asia Pac J Ophthalmol (Phila). 2015;4(2):101-105. doi:10.1097/APO.0000000000000085

  44. Kamoshita M, Matsumoto Y, Nishimura K, Katono Y, Murata M, Ozawa Y, Shimmura S, Tsubota K. Wickerhamomyces anomalus fungal keratitis responds to topical treatment with antifungal micafungin. J Infect Chemother. 2015;21(2):141-143. doi:10.1016/j.jiac.2014.07.011

  45. Fukui M, Ogawa Y, Shimmura S, Hatou S, Ichihashi Y, Yaguchi S, Hirayama M, Kawakita T, Tsubota K. Possible involvement of epithelial-mesenchymal transition in fibrosis associated with IgG4-related Mikulicz’s disease. Mod Rheumatol. 2015;25(5):811-817. doi:10.3109/14397595.2014.992224

  46. Yoshida T, Ozawa Y, Suzuki K, Yuki K, Ohyama M, Akamatsu W, Matsuzaki Y, Shimmura S, Mitani K, Tsubota K, Okano H. The use of induced pluripotent stem cells to reveal pathogenic gene mutations and explore treatments for retinitis pigmentosa. Mol Brain. 2014;7:45. doi:10.1186/1756-6606-7-45

  47. Shiba D, Hatou S, Ono T, Hosoda S, Tanabe S, Ozeki N, Yuki K, Shimoyama M, Fukagawa K, Shimmura S, Tsubota K. Multivariable logistic regression model: a novel mathematical model that predicts visual field sensitivity from macular ganglion cell complex thickness in glaucoma. PLoS One. 2014;9(8):e104126. doi:10.1371/journal.pone.0104126

  48. Satake Y, Yamaguchi T, Hirayama M, Higa K, Shimazaki-Den S, Dogru M, Kawakita T, Kawashima M, Shimmura S, Tsubota K, Shimazaki J. Ocular surface reconstruction by cultivated epithelial sheet transplantation. Cornea. 2014;33 Suppl 11:S42-46. doi:10.1097/ICO.0000000000000216

  49. Kamoshita M, Ozawa Y, Kubota S, Miyake S, Tsuda C, Nagai N, Yuki K, Shimmura S, Umezawa K, Tsubota K. AMPK-NF-kappaB axis in the photoreceptor disorder during retinal inflammation. PLoS One. 2014;9(7):e103013. doi:10.1371/journal.pone.0103013

  50. Fujii S, Matsumoto Y, Fukui M, Fujitake J, Kawakita T, Shimmura S, Tsubota K. Clinical backgrounds of postoperative keratoplasty patients with spontaneous wound dehiscence or gaps after suture removal. Cornea. 2014;33(12):1320-1323. doi:10.1097/ICO.0000000000000259

  51. Yaguchi S, Ogawa Y, Shimmura S, Kawakita T, Hatou S, Satofuka S, Nakamura S, Imada T, Miyashita H, Yoshida S, Yaguchi T, Ozawa Y, Mori T, Okamoto S, Kawakami Y, Ishida S, Tsubota K. Angiotensin II type 1 receptor antagonist attenuates lacrimal gland, lung, and liver fibrosis in a murine model of chronic graft-versus-host disease. PLoS One. 2013;8(6):e64724. doi:10.1371/journal.pone.0064724

  52. Narimatsu T, Ozawa Y, Miyake S, Kubota S, Hirasawa M, Nagai N, Shimmura S, Tsubota K. Disruption of cell-cell junctions and induction of pathological cytokines in the retinal pigment epithelium of light-exposed mice. Invest Ophthalmol Vis Sci. 2013;54(7):4555-4562. doi:10.1167/iovs.13-11883

  53. Miyashita H, Yokoo S, Yoshida S, Kawakita T, Yamagami S, Tsubota K, Shimmura S. Long-term maintenance of limbal epithelial progenitor cells using rho kinase inhibitor and keratinocyte growth factor. Stem Cells Transl Med. 2013;2(10):758-765. doi:10.5966/sctm.2013-0010

  54. Konomi K, Satake Y, Shimmura S, Tsubota K, Shimazaki J. Long-term results of amniotic membrane transplantation for partial limbal deficiency. Cornea. 2013;32(8):1110-1115. doi:10.1097/ICO.0b013e318288d227

  55. Kawakita T, Kawabata F, Tsuji T, Kawashima M, Shimmura S, Tsubota K. Effects of dietary supplementation with fish oil on dry eye syndrome subjects: randomized controlled trial. Biomed Res. 2013;34(5):215-220. doi:10.2220/biomedres.34.215

  56. Kawai M, Ogawa Y, Shimmura S, Ohta S, Suzuki T, Kawamura N, Kuwana M, Kawakami Y, Tsubota K. Expression and localization of aging markers in lacrimal gland of chronic graft-versus-host disease. Sci Rep. 2013;3:2455. doi:10.1038/srep02455

  57. Inagaki E, Hatou S, Yoshida S, Miyashita H, Tsubota K, Shimmura S. Expression and distribution of claudin subtypes in human corneal endothelium. Invest Ophthalmol Vis Sci. 2013;54(12):7258-7265. doi:10.1167/iovs.13-12502

  58. Hirayama M, Ogawa M, Oshima M, Sekine Y, Ishida K, Yamashita K, Ikeda K, Shimmura S, Kawakita T, Tsubota K, Tsuji T. Functional lacrimal gland regeneration by transplantation of a bioengineered organ germ. Nat Commun. 2013;4:2497. doi:10.1038/ncomms3497

  59. Higa K, Kato N, Yoshida S, Ogawa Y, Shimazaki J, Tsubota K, Shimmura S. Aquaporin 1-positive stromal niche-like cells directly interact with N-cadherin-positive clusters in the basal limbal epithelium. Stem Cell Res. 2013;10(2):147-155. doi:10.1016/j.stemcr.2012.11.006

  60. Hatou S, Yoshida S, Higa K, Miyashita H, Inagaki E, Okano H, Tsubota K, Shimmura S. Functional corneal endothelium derived from corneal stroma stem cells of neural crest origin by retinoic acid and Wnt/beta-catenin signaling. Stem Cells Dev. 2013;22(5):828-839. doi:10.1089/scd.2012.0305

  61. Hatou S, Higa K, Inagaki E, Yoshida S, Kimura E, Hayashi R, Tsujikawa M, Tsubota K, Nishida K, Shimmura S. Validation of Na,K-ATPase pump function of corneal endothelial cells for corneal regenerative medicine. Tissue Eng Part C Methods. 2013;19(12):901-910. doi:10.1089/ten.tec.2012.0725

  62. Yaguchi S, Ogawa Y, Shimmura S, Hatou S, Nakamura S, Inaba T, Imada T, Ozawa Y, Kawakami Y, Ishida S, Tsubota K. Presence and physiologic function of the renin-angiotensin system in mouse lacrimal gland. Invest Ophthalmol Vis Sci. 2012;53(9):5416-5425. doi:10.1167/iovs.12-9844

  63. Uchino Y, Kawakita T, Miyazawa M, Ishii T, Onouchi H, Yasuda K, Ogawa Y, Shimmura S, Ishii N, Tsubota K. Oxidative stress induced inflammation initiates functional decline of tear production. PLoS One. 2012;7(10):e45805. doi:10.1371/journal.pone.0045805

  64. Tatematsu Y, Ogawa Y, Shimmura S, Dogru M, Yaguchi S, Nagai T, Yamazaki K, Kameyama K, Okamoto S, Kawakami Y, Tsubota K. Mucosal microvilli in dry eye patients with chronic GVHD. Bone Marrow Transplant. 2012;47(3):416-425. doi:10.1038/bmt.2011.96

  65. Ozeki N, Yuki K, Shiba D, Shimmura S, Murat D, Tsubota K. Intraocular pressure elevation after Descemet’s stripping endothelial keratoplasty. Jpn J Ophthalmol. 2012;56(4):307-311. doi:10.1007/s10384-012-0164-8

  66. Omoto M, Yoshida S, Miyashita H, Kawakita T, Yoshida K, Kishino A, Kimura T, Shibata S, Tsubota K, Okano H, Shimmura S. The semaphorin 3A inhibitor SM-345431 accelerates peripheral nerve regeneration and sensitivity in a murine corneal transplantation model. PLoS One. 2012;7(11):e47716. doi:10.1371/journal.pone.0047716

  67. Kobayashi S, Kawakita T, Kawashima M, Okada N, Mishima K, Saito I, Ito M, Shimmura S, Tsubota K. Characterization of cultivated murine lacrimal gland epithelial cells. Mol Vis. 2012;18:1271-1277.

  68. Kawashima M, Kawakita T, Inaba T, Okada N, Ito M, Shimmura S, Watanabe M, Shinmura K, Tsubota K. Dietary lactoferrin alleviates age-related lacrimal gland dysfunction in mice. PLoS One. 2012;7(3):e33148. doi:10.1371/journal.pone.0033148

  69. Kamoi M, Ogawa Y, Nakamura S, Dogru M, Nagai T, Obata H, Ito M, Kaido M, Kawakita T, Okada Y, Kawakami Y, Shimmura S, Tsubota K. Accumulation of secretory vesicles in the lacrimal gland epithelia is related to non-Sjogren’s type dry eye in visual display terminal users. PLoS One. 2012;7(9):e43688. doi:10.1371/journal.pone.0043688

  70. Yoshida S, Yasuda M, Miyashita H, Ogawa Y, Yoshida T, Matsuzaki Y, Tsubota K, Okano H, Shimmura S. Generation of stratified squamous epithelial progenitor cells from mouse induced pluripotent stem cells. PLoS One. 2011;6(12):e28856. doi:10.1371/journal.pone.0028856

  71. Uchino Y, Shimmura S, Yamaguchi T, Kawakita T, Matsumoto Y, Negishi K, Tsubota K. Comparison of corneal thickness and haze in DSAEK and penetrating keratoplasty. Cornea. 2011;30(3):287-290. doi:10.1097/ICO.0b013e3181f08535

  72. Shimazaki J, Den S, Omoto M, Satake Y, Shimmura S, Tsubota K. Prospective, randomized study of the efficacy of systemic cyclosporine in high-risk corneal transplantation. Am J Ophthalmol. 2011;152(1):33-39.e1. doi:10.1016/j.ajo.2011.01.019

  73. Okada N, Kawakita T, Mishima K, Saito I, Miyashita H, Yoshida S, Shimmura S, Tsubota K. Clusterin promotes corneal epithelial cell growth through upregulation of hepatocyte growth factor by mesenchymal cells in vitro. Invest Ophthalmol Vis Sci. 2011;52(6):2905-2910. doi:10.1167/iovs.10-6712

  74. Niibe K, Kawamura Y, Araki D, Morikawa S, Miura K, Suzuki S, Shimmura S, Sunabori T, Mabuchi Y, Nagai Y, Nakagawa T, Okano H, Matsuzaki Y. Purified mesenchymal stem cells are an efficient source for iPS cell induction. PLoS One. 2011;6(3):e17610. doi:10.1371/journal.pone.0017610

  75. Kubota M, Shimmura S, Kubota S, Miyashita H, Kato N, Noda K, Ozawa Y, Usui T, Ishida S, Umezawa K, Kurihara T, Tsubota K. Hydrogen and N-acetyl-L-cysteine rescue oxidative stress-induced angiogenesis in a mouse corneal alkali-burn model. Invest Ophthalmol Vis Sci. 2011;52(1):427-433. doi:10.1167/iovs.10-5807

  76. Kawashima M, Kawakita T, Maida Y, Kamoi M, Ogawa Y, Shimmura S, Masutomi K, Tsubota K. Comparison of telomere length and association with progenitor cell markers in lacrimal gland between Sjogren syndrome and non-Sjogren syndrome dry eye patients. Mol Vis. 2011;17:1397-1404.

  77. Kawakita T, Higa K, Shimmura S, Tomita M, Tsubota K, Shimazaki J. Fate of corneal epithelial cells separated from limbus in vivo. Invest Ophthalmol Vis Sci. 2011;52(11):8132-8137. doi:10.1167/iovs.11-7667

  78. Inagaki E, Ogawa Y, Matsumoto Y, Kawakita T, Shimmura S, Tsubota K. Four cases of corneal perforation in patients with chronic graft-versus-host disease. Mol Vis. 2011;17:598-606.

  79. Higa K, Takeshima N, Moro F, Kawakita T, Kawashima M, Demura M, Shimazaki J, Asakura T, Tsubota K, Shimmura S. Porous Silk Fibroin Film as a Transparent Carrier for Cultivated Corneal Epithelial Sheets. J Biomater Sci. 2011;22(16):2261-2276. doi:10.1163/156856211X562723

  80. Hatou S, Shimmura S, Shimazaki J, Usui T, Amano S, Yokogawa H, Kobayashi A, Zheng X, Shiraishi A, Ohashi Y, Inatomi T, Tsubota K. Mathematical projection model of visual loss due to fuchs corneal dystrophy. Invest Ophthalmol Vis Sci. 2011;52(11):7888-7893. doi:10.1167/iovs.11-7788

  81. Hatou S, Dogru M, Ibrahim OM, Wakamatsu T, Sato EA, Shimmura S, Negishi K, Tsubota K. The application of in vivo confocal scanning laser microscopy in the diagnosis and evaluation of treatment responses in Mooren’s ulcer. Invest Ophthalmol Vis Sci. 2011;52(9):6680-6689. doi:10.1167/iovs.11-7666

  82. Yamaguchi T, Negishi K, Yamaguchi K, Dogru M, Uchino Y, Shimmura S, Tsubota K. Comparison of anterior and posterior corneal surface irregularity in Descemet stripping automated endothelial keratoplasty and penetrating keratoplasty. Cornea. 2010;29(10):1086-1090. doi:10.1097/ICO.0b013e3181d9f07a

  83. Omoto M, Shimmura S, Hatou S, Ichihashi Y, Kawakita T, Tsubota K. Simultaneous deep anterior lamellar keratoplasty and limbal allograft in bilateral limbal stem cell deficiency. Jpn J Ophthalmol. 2010;54(6):537-543. doi:10.1007/s10384-010-0870-1

  84. Kubota M, Shimmura S, Miyashita H, Kawashima M, Kawakita T, Tsubota K. The anti-oxidative role of ABCG2 in corneal epithelial cells. Invest Ophthalmol Vis Sci. 2010;51(11):5617-5622. doi:10.1167/iovs.10-5201

  85. Kawashima M, Kawakita T, Okada N, Ogawa Y, Murat D, Nakamura S, Nakashima H, Shimmura S, Shinmura K, Tsubota K. Calorie restriction: A new therapeutic intervention for age-related dry eye disease in rats. Biochem Biophys Res Commun. 2010;397(4):724-728. doi:10.1016/j.bbrc.2010.06.050

  86. Yuki K, Shimmura S, Shiba D, Tsubota K. End-stage glaucoma in Stevens-Johnson syndrome. Jpn J Ophthalmol. 2009;53(1):68-70. doi:10.1007/s10384-008-0610-8

  87. Yamaguchi T, Negishi K, Yamaguchi K, Murat D, Uchino Y, Shimmura S, Tsubota K. Effect of anterior and posterior corneal surface irregularity on vision after Descemet-stripping endothelial keratoplasty. J Cataract Refract Surg. 2009;35(4):688-694. doi:10.1016/j.jcrs.2008.12.029

  88. Omoto M, Miyashita H, Shimmura S, Higa K, Kawakita T, Yoshida S, McGrogan M, Shimazaki J, Tsubota K. The use of human mesenchymal stem cell-derived feeder cells for the cultivation of transplantable epithelial sheets. Invest Ophthalmol Vis Sci. 2009;50(5):2109-2115. doi:10.1167/iovs.08-2895

  89. Ogawa Y, Shimmura S, Kawakita T, Yoshida S, Kawakami Y, Tsubota K. Epithelial mesenchymal transition in human ocular chronic graft-versus-host disease. Am J Pathol. 2009;175(6):2372-2381. doi:10.2353/ajpath.2009.090333

  90. Morikawa S, Mabuchi Y, Niibe K, Suzuki S, Nagoshi N, Sunabori T, Shimmura S, Nagai Y, Nakagawa T, Okano H, Matsuzaki Y. Development of mesenchymal stem cells partially originate from the neural crest. Biochem Biophys Res Commun. 2009;379(4):1114-1119. doi:10.1016/j.bbrc.2008.12.164

  91. Morikawa S, Mabuchi Y, Kubota Y, Nagai Y, Niibe K, Hiratsu E, Suzuki S, Miyauchi-Hara C, Nagoshi N, Sunabori T, Shimmura S, Miyawaki A, Nakagawa T, Suda T, Okano H, Matsuzaki Y. Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow. J Exp Med. 2009;206(11):2483-2496. doi:10.1084/jem.20091046

  92. Ma X, Shimmura S, Miyashita H, Yoshida S, Kubota M, Kawakita T, Tsubota K. Long-term culture and growth kinetics of murine corneal epithelial cells expanded from single corneas. Invest Ophthalmol Vis Sci. 2009;50(6):2716-2721. doi:10.1167/iovs.08-3165

  93. Kurihara T, Omoto M, Noda K, Ebinuma M, Kubota S, Koizumi H, Yoshida S, Ozawa Y, Shimmura S, Ishida S, Tsubota K. Retinal phototoxicity in a novel murine model of intraocular lens implantation. Mol Vis. 2009;15:2751-2761.

  94. Kawashima M, Kawakita T, Yoshida S, Shimmura S, Tsubota K. Nucleostemin as a possible progenitor marker of corneal epithelial cells. Mol Vis. 2009;15:1162-1168.

  95. Kawashima M, Kawakita T, Shimmura S, Tsubota K, Shimazaki J. Characteristics of traumatic globe rupture after keratoplasty. Ophthalmology. 2009;116(11):2072-2076. doi:10.1016/j.ophtha.2009.05.011

  96. Kawakita T, Shimmura S, Higa K, Espana EM, He H, Shimazaki J, Tsubota K, Tseng SC. Greater growth potential of p63-positive epithelial cell clusters maintained in human limbal epithelial sheets. Invest Ophthalmol Vis Sci. 2009;50(10):4611-4617. doi:10.1167/iovs.08-2894

  97. Higa K, Shimmura S, Miyashita H, Kato N, Ogawa Y, Kawakita T, Shimazaki J, Tsubota K. N-cadherin in the maintenance of human corneal limbal epithelial progenitor cells in vitro. Invest Ophthalmol Vis Sci. 2009;50(10):4640-4645. doi:10.1167/iovs.08-3199

  98. Yuki K, Shimmura S, Shiba D, Tsubota K. Trabeculotomy for the treatment of glaucoma after Descemet stripping endothelial keratoplasty. Br J Ophthalmol. 2008;92(9):1299-1300. doi:10.1136/bjo.2008.141872

  99. Uchino M, Schaumberg DA, Dogru M, Uchino Y, Fukagawa K, Shimmura S, Satoh T, Takebayashi T, Tsubota K. Prevalence of dry eye disease among Japanese visual display terminal users. Ophthalmology. 2008;115(11):1982-1988. doi:10.1016/j.ophtha.2008.05.022

  100. Uchino M, Dogru M, Uchino Y, Fukagawa K, Shimmura S, Takebayashi T, Schaumberg DA, Tsubota K. Japan Ministry of Health study on prevalence of dry eye disease among Japanese high school students. Am J Ophthalmol. 2008;146(6):925-929.e2. doi:10.1016/j.ajo.2008.07.019

  101. Tomita M, Shimmura S, Tsubota K, Shimazaki J. Postkeratoplasty atopic sclerokeratitis in keratoconus patients. Ophthalmology. 2008;115(5):851-856. doi:10.1016/j.ophtha.2007.07.036

  102. Ozawa Y, Nakao K, Kurihara T, Shimazaki T, Shimmura S, Ishida S, Yoshimura A, Tsubota K, Okano H. Roles of STAT3/SOCS3 pathway in regulating the visual function and ubiquitin-proteasome-dependent degradation of rhodopsin during retinal inflammation. J Biol Chem. 2008;283(36):24561-24570. doi:10.1074/jbc.M802871200

  103. Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, Ishida S. Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. Invest Ophthalmol Vis Sci. 2008;49(5):2172-2177. doi:10.1167/iovs.07-1335

  104. Miyashita H, Shimmura S, Higa K, Yoshida S, Kawakita T, Shimazaki J, Tsubota K. A novel NIH/3T3 duplex feeder system to engineer corneal epithelial sheets with enhanced cytokeratin 15-positive progenitor populations. Tissue Eng Part A. 2008;14(7):1275-1282. doi:10.1089/ten.tea.2007.0371

  105. Kawakita T, Shimmura S, Hornia A, Higa K, Tseng SC. Stratified epithelial sheets engineered from a single adult murine corneal/limbal progenitor cell. J Cell Mol Med. 2008;12(4):1303-1316. doi:10.1111/j.1582-4934.2008.00160.x

  106. Igarashi T, Shimmura S, Yoshida S, Tonogi M, Shinozaki N, Yamane GY. Isolation of oral epithelial progenitors using collagen IV. Oral Dis. 2008;14(5):413-418. doi:10.1111/j.1601-0825.2007.01391.x

  107. Uchino Y, Shimmura S, Miyashita H, Taguchi T, Kobayashi H, Shimazaki J, Tanaka J, Tsubota K. Amniotic membrane immobilized poly(vinyl alcohol) hybrid polymer as an artificial cornea scaffold that supports a stratified and differentiated corneal epithelium. J Biomed Mater Res B Appl Biomater. 2007;81(1):201-206. doi:10.1002/jbm.b.30653

  108. Shimazaki J, Higa K, Morito F, Dogru M, Kawakita T, Satake Y, Shimmura S, Tsubota K. Factors influencing outcomes in cultivated limbal epithelial transplantation for chronic cicatricial ocular surface disorders. Am J Ophthalmol. 2007;143(6):945-953. doi:10.1016/j.ajo.2007.02.043

  109. Ozawa Y, Nakao K, Shimazaki T, Shimmura S, Kurihara T, Ishida S, Yoshimura A, Tsubota K, Okano H. SOCS3 is required to temporally fine-tune photoreceptor cell differentiation. Dev Biol. 2007;303(2):591-600. doi:10.1016/j.ydbio.2006.12.022

  110. Ogawa Y, Razzaque MS, Kameyama K, Hasegawa G, Shimmura S, Kawai M, Okamoto S, Ikeda Y, Tsubota K, Kawakami Y, Kuwana M. Role of heat shock protein 47, a collagen-binding chaperone, in lacrimal gland pathology in patients with cGVHD. Invest Ophthalmol Vis Sci. 2007;48(3):1079-1086. doi:10.1167/iovs.06-0857

  111. Miyashita H, Higa K, Kato N, Kawakita T, Yoshida S, Tsubota K, Shimmura S. Hypoxia enhances the expansion of human limbal epithelial progenitor cells in vitro. Invest Ophthalmol Vis Sci. 2007;48(8):3586-3593. doi:10.1167/iovs.07-0130

  112. Matsumoto Y, Dogru M, Sato EA, Katono Y, Uchino Y, Shimmura S, Tsubota K. The application of in vivo confocal scanning laser microscopy in the management of Acanthamoeba keratitis. Mol Vis. 2007;13:1319-1326.

  113. Kato N, Shimmura S, Kawakita T, Miyashita H, Ogawa Y, Yoshida S, Higa K, Okano H, Tsubota K. Beta-catenin activation and epithelial-mesenchymal transition in the pathogenesis of pterygium. Invest Ophthalmol Vis Sci. 2007;48(4):1511-1517. doi:10.1167/iovs.06-0562

  114. Higuchi A, Ueno R, Shimmura S, Suematsu M, Dogru M, Tsubota K. Albumin rescues ocular epithelial cells from cell death in dry eye. Curr Eye Res. 2007;32(2):83-88. doi:10.1080/02713680601111003

  115. Higa K, Shimmura S, Kato N, Kawakita T, Miyashita H, Itabashi Y, Fukuda K, Shimazaki J, Tsubota K. Proliferation and Differentiation of Transplantable Rabbit Epithelial Sheets Engineered with or without an Amniotic Membrane Carrier. Invest Ophthalmol Vis Sci. 2007;48(2):597-604. doi:10.1167/iovs.06-0414

  116. Dogru M, Kato N, Matsumoto Y, Tanaka Y, Akabane N, Shimmura S, Tsubota K, Shimazaki J. Immunohistochemistry and electron microscopy of retrocorneal scrolls in syphilitic interstitial keratitis. Curr Eye Res. 2007;32(10):863-870. doi:10.1080/02713680701625409

  117. Den S, Shimmura S, Tsubota K, Shimazaki J. Impact of the descemet membrane perforation on surgical outcomes after deep lamellar keratoplasty. Am J Ophthalmol. 2007;143(5):750-754. doi:10.1016/j.ajo.2007.01.009

  118. Yoshida S, Shimmura S, Nagoshi N, Fukuda K, Matsuzaki Y, Okano H, Tsubota K. Isolation of multipotent neural crest-derived stem cells from the adult mouse cornea. Stem Cells. 2006;24(12):2714-2722. doi:10.1634/stemcells.2006-0275

  119. Yoshida S, Shimmura S, Kawakita T, Miyashita H, Den S, Shimazaki J, Tsubota K. Cytokeratin 15 can be used to identify the limbal phenotype in normal and diseased ocular surfaces. Invest Ophthalmol Vis Sci. 2006;47(11):4780-4786. doi:10.1167/iovs.06-0371

  120. Uchino Y, Goto E, Takano Y, Dogru M, Shinozaki N, Shimmura S, Yagi Y, Tsubota K, Shimazaki J. Long-standing bullous keratopathy is associated with peripheral conjunctivalization and limbal deficiency. Ophthalmology. 2006;113(7):1098-1101. doi:10.1016/j.ophtha.2006.01.077

  121. Tomita M, Shimmura S, Tsubota K, Shimazaki J. Dacryoadenitis associated with Acanthamoeba keratitis. Arch Ophthalmol. 2006;124(9):1239-1242. doi:10.1001/archopht.124.9.1239

  122. Shimmura S, Miyashita H, Uchino Y, Taguchi T, Kobayashi H, Shimazaki J, Tanaka J, Tsubota K. Microkeratome assisted deep lamellar keratoprosthesis. Br J Ophthalmol. 2006;90(7):826-829. doi:10.1136/bjo.2006.090624

  123. Shimmura S, Miyashita H, Higa K, Yoshida S, Shimazaki J, Tsubota K. Proteomic analysis of soluble factors secreted by limbal fibroblasts. Mol Vis. 2006;12:478-484.

  124. Shimazaki J, Konomi K, Shimmura S, Tsubota K. Ocular surface reconstruction for thermal burns caused by fireworks. Cornea. 2006;25(2):139-145. doi:10.1097/01.ico.0000179979.62024.47

  125. Miyashita H, Shimmura S, Kobayashi H, Taguchi T, Asano-Kato N, Uchino Y, Kato M, Shimazaki J, Tanaka J, Tsubota K. Collagen-immobilized poly(vinyl alcohol) as an artificial cornea scaffold that supports a stratified corneal epithelium. J Biomed Mater Res B Appl Biomater. 2006;76(1):56-63. doi:10.1002/jbm.b.30325

  126. Maruyama-Hosoi F, Shimazaki J, Shimmura S, Tsubota K. Changes Observed in Keratolimbal Allograft. Cornea. 2006;25(4):377-382. doi:10.1097/01.ico.0000213133.05739.0a

  127. Kawashima M, Kawakita T, Den S, Shimmura S, Tsubota K, Shimazaki J. Comparison of deep lamellar keratoplasty and penetrating keratoplasty for lattice and macular corneal dystrophies. Am J Ophthalmol. 2006;142(2):304-309. doi:10.1016/j.ajo.2006.03.041

  128. Higuchi A, Shimmura S, Takeuchi T, Suematsu M, Tsubota K. Elucidation of apoptosis induced by serum deprivation in cultured conjunctival epithelial cells. Br J Ophthalmol. 2006;90(6):760-764. doi:10.1136/bjo.2005.083892

  129. Higa K, Shimmura S, Shimazaki J, Tsubota K. Ocular surface epithelial cells up-regulate HLA-G when expanded in vitro on amniotic membrane substrates. Cornea. 2006;25(6):715-721. doi:10.1097/01.ico.0000220666.27361.64

  130. Den S, Shimizu K, Ikeda T, Tsubota K, Shimmura S, Shimazaki J. Association between meibomian gland changes and aging, sex, or tear function. Cornea. 2006;25(6):651-655. doi:10.1097/01.ico.0000220664.12027.66

  131. Den S, Dogru M, Kato N, Tanaka Y, Shimmura S, Shimazaki J. Disseminated keratoconjunctival epitheliopathy in a patient with systemic lupus erythematosus. Br J Ophthalmol. 2006;90(8):1068-1070. doi:10.1136/bjo.2006.091010

  132. Yoshida S, Shimmura S, Shimazaki J, Shinozaki N, Tsubota K. Serum-free spheroid culture of mouse corneal keratocytes. Invest Ophthalmol Vis Sci. 2005;46(5):1653-1658. doi:10.1167/iovs.04-1249

  133. Shimmura S, Shimazaki J, Omoto M, Teruya A, Ishioka M, Tsubota K. Deep lamellar keratoplasty (DLKP) in keratoconus patients using viscoadaptive viscoelastics. Cornea. 2005;24(2):178-181. doi:10.1097/01.ico.0000147660.10984.c3

  134. Shimmura S, Omoto M, Den S, Bissen-Miyajima H, Tsubota K, Shimazaki J. Microkeratome-assisted phacoemulsification. J Cataract Refract Surg. 2005;31(9):1699-1701. doi:10.1016/j.jcrs.2005.05.029

  135. Shimmura S, Miyashita H, Konomi K, Shinozaki N, Taguchi T, Kobayashi H, Shimazaki J, Tanaka J, Tsubota K. Transplantation of corneal endothelium with Descemet’s membrane using a hyroxyethyl methacrylate polymer as a carrier. Br J Ophthalmol. 2005;89(2):134-137. doi:10.1136/bjo.2004.053523

  136. Higa K, Shimmura S, Shimazaki J, Tsubota K. Hyaluronic acid-CD44 interaction mediates the adhesion of lymphocytes by amniotic membrane stroma. Cornea. 2005;24(2):206-212. doi:10.1097/01.ico.0000148408.82421.36

  137. Higa K, Shimmura S, Miyashita H, Shimazaki J, Tsubota K. Melanocytes in the corneal limbus interact with K19-positive basal epithelial cells. Exp Eye Res. 2005;81(2):218-223. doi:10.1016/j.exer.2005.02.007

  138. Dogru M, Asano-Kato N, Tanaka M, Igarashi A, Shimmura S, Shimazaki J, Okada N, Takano Y, Fukagawa K, Tsubota K, Fujishima H. Ocular Surface and MUC5AC Alterations in Atopic Patients with Corneal Shield Ulcers. Curr Eye Res. 2005;30(10):897-908. doi:10.1080/02713680500305886

  139. Shimmura S, Tadano K, Tsubota K. UV dose-dependent caspase activation in a corneal epithelial cell line. Curr Eye Res. 2004;28(2):85-92. doi:10.1076/ceyr.28.2.85.25304

  140. Shimmura S, Ando M, Ishioka M, Shimazaki J, Tsubota K. Same-size donor corneas for myopic keratoconus. Cornea. 2004;23(4):345-349. doi:10.1097/01.ico.0000109968.41434.b0

  141. Shimazaki J, Shinozaki N, Shimmura S, Holland EJ, Tsubota K. Efficacy and safety of international donor sharing: a single-center, case-controlled study on corneal transplantation. Transplantation. 2004;78(2):216-220. doi:10.1097/01.tp.0000127598.67975.d4

  142. Shimazaki J, Shimmura S, Tsubota K. Donor source affects the outcome of ocular surface reconstruction in chemical or thermal burns of the cornea. Ophthalmology. 2004;111(1):38-44. doi:10.1016/j.ophtha.2003.05.004

  143. Konomi K, Shimazaki J, Shimmura S, Akabane N, Goto E, Tsubota K. Efficacy of core vitrectomy preceding triple corneal procedure. Br J Ophthalmol. 2004;88(8):1023-1025. doi:10.1136/bjo.2003.037419

  144. Kobayashi H, Kato M, Taguchi T, Ikoma T, Miyashita H, Shimmura S, Tsubota K, Tanaka J. Collagen immobilized PVA hydrogel-hydroxyapatite composites prepared by kneading methods as a material for peripheral cuff of artificial cornea. Mater Sci Eng C. 2004;24(5):729-735. doi:10.1016/j.msec.2004.02.004

  145. Dogru M, Kaderli B, Gelisken O, Yucel A, Avci R, Goto E, Shimmura S, Shimazaki J, Tsubota K. Ocular surface changes with applanation contact lens and coupling fluid use after argon laser photocoagulation in noninsulin-dependent diabetes mellitus. Am J Ophthalmol. 2004;138(3):381-388. doi:10.1016/j.ajo.2004.05.006

  146. Bissen-Miyajima H, Nakamura K, Kaido M, Shimmura S, Tsubota K. Role of the endothelial pump in flap adhesion after laser in situ keratomileusis. J Cataract Refract Surg. 2004;30(9):1989-1992. doi:10.1016/j.jcrs.2004.04.028

  147. Shimmura S, Ueno R, Matsumoto Y, Goto E, Higuchi A, Shimazaki J, Tsubota K. Albumin as a tear supplement in the treatment of severe dry eye. Br J Ophthalmol. 2003;87(10):1279-1283. doi:10.1136/bjo.87.10.1279

  148. Shimmura S, Shimazaki J, Tsubota K. Therapeutic deep lamellar keratoplasty for cornea perforation. Am J Ophthalmol. 2003;135(6):896-897. doi:10.1016/s0002-9394(03)00150-7

  149. Shimmura S, Ohashi Y, Shiroma H, Shimazaki J, Tsubota K. Corneal opacity and cataract: triple procedure versus secondary approach. Cornea. 2003;22(3):234-238. doi:10.1097/00003226-200304000-00008

  150. Shimmura S, Igarashi R, Yaguchi H, Ohashi Y, Shimazaki J, Tsubota K. Lecithin-bound superoxide dismutase in the treatment of noninfectious corneal ulcers. Am J Ophthalmol. 2003;135(5):613-619. doi:10.1016/s0002-9394(02)02242-4

  151. Shimmura S, Doillon CJ, Griffith M, Nakamura M, Gagnon E, Usui A, Shinozaki N, Tsubota K. Collagen-poly(N-isopropylacrylamide)-based membranes for corneal stroma scaffolds. Cornea. 2003;22(7 Suppl):S81-S88. doi:10.1097/00003226-200310001-00014

  152. Shimazaki J, Kosaka K, Shimmura S, Tsubota K. Amniotic membrane transplantation with conjunctival autograft for recurrent pterygium. Ophthalmology. 2003;110(1):119-124. doi:10.1016/S0161-6420(02)01540-X

  153. Mori A, Shimazaki J, Shimmura S, Fujishima H, Oguchi Y, Tsubota K. Disposable eyelid-warming device for the treatment of meibomian gland dysfunction. Jpn J Ophthalmol. 2003;47(6):578-586. doi:10.1016/j.jjo.2003.08.006

  154. Asano-Kato N, Toda I, Shimmura S, Noda-Tsuruya T, Fukagawa K, Yoshinaga M, Matsukawa A, Tsubota K. Detection of neutrophils and possible involvement of interleukin-8 in diffuse lamellar keratitis after laser in situ keratomileusis. J Cataract Refract Surg. 2003;29(10):1996-2000. doi:10.1016/S0886-3350(03)00474-0

  155. Tsubota K, Shimmura S, Shinozaki N, Holland EJ, Shimazaki J. Clinical application of living-related conjunctival-limbal allograft. Am J Ophthalmol. 2002;133(1):134-135. doi:10.1016/S0002-9394(01)01264-9

  156. Takahashi H, Sakamoto A, Takahashi R, Ohmura T, Shimmura S, Ohara K. Free radicals in phacoemulsification and aspiration procedures. Arch Ophthalmol. 2002;120(10):1348-1352. doi:10.1001/archopht.120.10.1348

  157. Shimazaki J, Shimmura S, Tsubota K. Limbal stem cell transplantation for the treatment of subepithelial amyloidosis of the cornea (gelatinous drop-like dystrophy). Cornea. 2002;21(2):177-180. doi:10.1097/00003226-200203000-00009

  158. Shimazaki J, Shimmura S, Ishioka M, Tsubota K. Randomized clinical trial of deep lamellar keratoplasty vs penetrating keratoplasty. Am J Ophthalmol. 2002;134(2):159-165. doi:10.1016/S0002-9394(02)01524-7

  159. Shimazaki J, Aiba M, Goto E, Kato N, Shimmura S, Tsubota K. Transplantation of human limbal epithelium cultivated on amniotic membrane for the treatment of severe ocular surface disorders. Ophthalmology. 2002;109(7):1285-1290. doi:10.1016/S0161-6420(02)01018-8

  160. Higuchi A, Shimmura S, Ishii M, Aburatani H, Tsubota K. Serum- and serum deprivation-induced transcriptional profiles of cultured conjunctival epithelial cells. Adv Exp Med Biol. 2002;506(Pt A):673-676.

  161. Goto E, Shimazaki J, Monden Y, Takano Y, Yagi Y, Shimmura S, Tsubota K. Low-concentration homogenized castor oil eye drops for noninflamed obstructive meibomian gland dysfunction. Ophthalmology. 2002;109(11):2030-2035. doi:10.1016/S0161-6420(02)01254-2

  162. Goto E, Monden Y, Takano Y, Mori A, Shimmura S, Shimazaki J, Tsubota K. Treatment of non-inflamed obstructive meibomian gland dysfunction by an infrared warm compression device. Br J Ophthalmol. 2002;86(12):1403-1407. doi:10.1136/bjo.86.12.1403

  163. Shimmura S, Shimazaki J, Ohashi Y, Tsubota K. Anti-inflammatory effects of amniotic membrane transplantation in ocular surface disorders. Cornea. 2001;20(4):408-413. doi:10.1097/00003226-200105000-00015

  164. Shimazaki J, Maruyama F, Shimmura S, Fujishima H, Tsubota K. Immunologic rejection of the central graft after limbal allograft transplantation combined with penetrating keratoplasty. Cornea. 2001;20(2):149-152. doi:10.1097/00003226-200103000-00008

  165. Miyoshi T, Fukagawa K, Shimmura S, Fujishima H, Takano Y, Takamura E, Tsubota K, Saito H, Oguchi Y. Interleukin-8 concentrations in conjunctival epithelium brush cytology samples correlate with neutrophil, eosinophil infiltration, and corneal damage. Cornea. 2001;20(7):743-747. doi:10.1097/00003226-200110000-00016

  166. Ishioka M, Shimmura S, Yagi Y, Tsubota K. Pterygium and dry eye. Ophthalmologica. 2001;215(3):209-211. doi:10.1159/000050868

  167. Hanada K, Shimazaki J, Shimmura S, Tsubota K. Multilayered amniotic membrane transplantation for severe ulceration of the cornea and sclera. Am J Ophthalmol. 2001;131(3):324-331. doi:10.1016/S0002-9394(00)00779-7

  168. Goto E, Shimmura S, Shimazaki J, Tsubota K. Treatment of superior limbic keratoconjunctivitis by application of autologous serum. Cornea. 2001;20(8):807-810. doi:10.1097/00003226-200111000-00005

  169. Shimmura S, Matsumoto K, Yaguchi H, Okuda T, Miyajima S, Negi A, Shimazaki J, Tsubota K. Acidic electrolyzed water in the disinfection of the ocular surface. Exp Eye Res. 2000;70(1):1-6. doi:10.1006/exer.1999.0782

  170. Shimmura S, Ishioka M, Hanada K, Shimazaki J, Tsubota K. Telomerase activity and p53 expression in pterygia. Invest Ophthalmol Vis Sci. 2000;41(6):1364-1369.

  171. Shimmura S, Ando M, Shimazaki J, Tsubota K. Complications with one-piece lamellar kerato-limbal grafts for simultaneous limbal and corneal pathologies. Cornea. 2000;19(4):439-442. doi:10.1097/00003226-200007000-00009

  172. Shimazaki S, Shimmura S, Fujishima H, Tsubota K. Association of preoperative tear function with surgical outcome in severe Stevens-Johnson syndrome. Ophthalmology. 2000;107(8):1518-1523. doi:10.1016/S0161-6420(00)00244-9

  173. Shimazaki J, Hanada K, Yagi Y, Yamagami J, Ishioka M, Shimmura S, Tsubota K. Changes in ocular surface caused by antiglaucomatous eyedrops: prospective, randomised study for the comparison of 0.5% timolol v 0. 12% unoprostone. Br J Ophthalmol. 2000;84(11):1250-1254. doi:10.1136/bjo.84.11.1250

  174. Nakamura K, Bissen-Miyajima H, Shimmura S, Tsubota K. Clinical application of Er:YAG laser for the treatment of pterygium. Ophthalmic Surg Lasers. 2000;31(1):8-12.

  175. Ishioka M, Shimazaki J, Yamagami J, Fujishima H, Shimmura S, Tsubota K. Trabeculectomy with mitomycin C for post-keratoplasty glaucoma. Br J Ophthalmol. 2000;84(7):714-717. doi:10.1136/bjo.84.7.714

  176. Fukagawa K, Nakajima T, Saito H, Tsubota K, Shimmura S, Natori M, Hirai K. IL-4 induces eotaxin production in corneal keratocytes but not in epithelial cells. Int Arch Allergy Immunol. 2000;121(2):144-150. doi:10.1159/000024340

  177. Tsubota K, Satake Y, Kaido M, Shinozaki N, Shimmura S, Bissen-Miyajima H, Shimazaki J. Treatment of severe ocular-surface disorders with corneal epithelial stem-cell transplantation. N Engl J Med. 1999;340(22):1697-1703. doi:10.1056/NEJM199906033402203

  178. Tsubota K, Monden Y, Yagi Y, Goto E, Shimmura S. New treatement of dry eye. The effect of calcium ointment through eyelid skin delivery. Br J Ophthalmol. 1999;83(6):767-770. doi:10.1136/bjo.83.6.767

  179. Tsubota K, Kaido M, Yagi Y, Fujihara T, Shimmura S. Diseases associated with ocular surface abnormalities: The importance of reflex tearing. Br J Ophthalmol. 1999;83(1):89-91. doi:10.1136/bjo.83.1.89

  180. Tsubota K, Goto E, Shimmura S, Shimazaki J. Treatment of persistent corneal epithelial defect by autologous serum application. Ophthalmology. 1999;106(10):1984-1989. doi:10.1016/S0161-6420(99)90415-X

  181. Tsubota K, Goto E, Fujita H, Ono M, Inoue H, Saito I, Shimmura S. Treatment of dry eye by autologus serum application in Sjogren’s Syndrome. Br J Ophthalmol. 1999;83(4):390-395. doi:10.1136/bjo.83.4.390

  182. Tsubota K, Fukagawa K, Fujihara T, Shimmura S, Saito I, Saito K, Takeuchi T. Regulation of HLA DR expression in human conjunctival epithelium. Invest Ophthalmol Vis Sci. 1999;40(1):28-34.

  183. Takano Y, Fukagawa K, Shimmura S, Tsubota K, Oguchi Y, Saito H. IL-4 regulates chemokine production induced by TNF-a in keratocytes and corneal epithelial cells. Br J Ophthalmol. 1999;83(9):1074-1076. doi:10.1136/bjo.83.9.1074

  184. Shimmura S, Shimazaki J, Tsubota K. Results of a population -based questionnaire on the symptoms and lifestyles associated with dry eye. Cornea. 1999;18(4):408-411. doi:10.1097/00003226-199907000-00009

  185. Shimmura S, Masumizu T, Urayama K, Shimazaki J, Bissen-Miyajima H, Tsubota K. Excimer laser-induced hydroxyl radical formation and keratocyte death in vitro. Invest Ophthalmol Vis Sci. 1999;40(6):1245-1249.

  186. Shimazaki J, Shimmura S, Yamato K, Tsubota K. Morphology and barrier function of the corneal epithelium following penetrating keratoplasty: association with original diseases, tear function, and suture removal. Cornea. 1999;18(5):559-564. doi:10.1097/00003226-199909000-00007

  187. Shimazaki J, Kaido M, Shinozaki N, Shimmura S, Munkhbat B, Hagihara M, Tsuji K, Tsubota K. Evidence of long-term survival of donor-derived cells after limbal allograft transplantation. Invest Ophthalmol Vis Sci. 1999;40(8):1664-1668.

  188. Nakamura K, Bissen-Miyajima H, Arai H, Toda I, Hori Y, Shimmura S, Tsubota K. Iatrogenic cataract after laser-assisted in situ keratomileusis. Am J Ophthalmol. 1999;128(4):507-509. doi:10.1016/S0002-9394(99)00216-7

  189. Fukagawa K, Nakajima T, Tsubota K, Shimmura S, Saito H, Hirai K. Presence of eotaxin in tears of patients with atopic keratoconjunctivitis with severe corneal damage. J Allergy Clin Immunol. 1999;103(6):1220-1221. doi:10.1016/S0091-6749(99)70267-3

  190. Bissen-Miyajima H, Shimmura S, Tsubota K. Thermal effect on corneal incisions with different ultrasonic tips. J Cataract Refract Surg. 1999;25(1):60-64. doi:10.1016/S0886-3350(99)80058-2

  191. Shimmura S, Shinozaki N, Fukagawa K, Shimazaki J, Tsubota K. Real-time telemedicine in the clinical assessment of the ocular surface. Am J Ophthalmol. 1998;125(3):388-390. doi:10.1016/S0002-9394(98)00344-7

  192. Shimmura S, Shimoyama M, Hojo M, Urayama K, Tsubota K. Reoxygenation injury in a cultured corneal epithelial cell line protected by the uptake of lactoferrin. Invest Ophthalmol Vis Sci. 1998;39(8):1346-1351.

  193. Shimmura S, Matsumoto K, Goto E, Saito K, Takeuchi T, Miyajima S, Negi A, Tsubota K. Lecithin-bound superoxide dismutase in the prevention of neutrophil-induced damage of corneal tissue. Invest Ophthalmol Vis Sci. 1998;39(1):30-35.

  194. Shimmura S, Goto E, Shimazaki J, Tsubota K. Viscosity-dependent fluid dynamics of eyedrops on the ocular surface. Am J Ophthalmol. 1998;125(3):386-388. doi:10.1016/S0002-9394(98)00343-5

  195. Shimazaki J, Yang HY, Shimmura S, Tsubota K. Terrien’s marginal degeneration associated with erythema elevatum diutinum. Cornea. 1998;17(3):342-344. doi:10.1097/00003226-199805000-00017

  196. Shimazaki J, Shimmura S, Tsubota K. Intraoperative versus postoperative suture adjustment after penetrating keratoplasty. Cornea. 1998;17(6):590-594. doi:10.1097/00003226-199811000-00014

  197. Shimazaki J, Goto E, Ono M, Shimmura S, Tsubota K. Meibomian gland dysfunction in patients with Sjogren syndrome. Ophthalmology. 1998;105(8):1485-1488. doi:10.1016/S0161-6420(98)98048-7

  198. Shimoyama M, Shimmura S, Tsubota K, Oguchi Y. Suppression of nuclear factor kappa B and CD 18 mediated leukocyte adhesion to the corneal endothelium by dexamethasone. Invest Ophthalmol Vis Sci. 1997;38(11):2427-2431.

  199. Shimmura S, Yang HY, Bissen-Miyajima H, Shimazaki J, Tsubota K. Posterior corneal protrusion following PRK. Cornea. 1997;16(6):686-688. doi:10.1097/00003226-199711000-00017

  200. Shimmura S, Tsubota K. Ultraviolet B-induced mitochondrial dysfunction is associated with decreased cell detachment of corneal epithelial cells in vitro. Invest Ophthalmol Vis Sci. 1997;38(3):620-626.

  201. Shimmura S, Shinozaki N, Fukagawa K, Tsubota K. Telemedicine in the follow-up of corneal transplant patients. J Telemed Telecare. 1997;3(4):227-228. doi:10.1258/1357633971931320

  202. Fukagawa K, Saito H, Tsubota K, Shimmura S, Tachimoto H, Akasawa A, Oguchi Y. RANTES production in a conjunctival epithelial cell line. Cornea. 1997;16(5):564-570.

  203. Shimmura S, Suematsu M, Shimoyama M, Tsubota K, Oguchi Y, Ishimura Y. Subthreshold UV radiation-induced peroxide formation in cultured corneal epithelial cells: the protective effects of lactoferrin. Exp Eye Res. 1996;63(5):519-526. doi:10.1006/exer.1996.0125

  204. Shimmura S, Ono M, Shinozaki K, Toda I, Takamura E, Mashima Y, Tsubota K. Sodium hyaluronate eyedrops in the treatment of dry eyes. Br J Ophthalmol. 1995;79(11):1007-1011. doi:10.1136/bjo.79.11.1007

  205. Shimmura S, Tsubota K, Oguchi Y, Fukumura D, Suematsu M, Tsuchiya M. Oxiradical-dependent photoemission induced by a phacoemulsification probe. Invest Ophthalmol Vis Sci. 1992;33(10):2904-2907.

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